Fig 1: Myeloid-specific MafB-deficient mice exhibit drastic changes in the proportion and phenotype of tissue RTMs.(A) Representative contour plots of MHC-II and F4/80 expression in peritoneal CD45+Lin−Ly6C−CD11b+CD115+ cells from Mafbfl/fl and Lyz2CreMafbfl/fl mice (left) and percentages of SPM and LPM, as gated on the contour plots (right) (n = 7–9). All subsequent panels compare Mafbfl/fl and Lyz2CreMafbfl/fl mice.(B) Absolute numbers of SPM and LPM, as in A.(C) Histograms (top) and bar graphs (bottom) of MHC-II and RELMα expression in SPM, as in A.(D) Histograms (top) and bar graphs (bottom) of F4/80, MerTK, Tim4, and Vsig4 expression in LPM, as in A.(E) Representative contour plots of F4/80 and CD11b expression in liver CD45+Lin−Ly6C− cells (left) and percentages of F4/80−CD11blo cells, CD11bhi Mo-Macs, and KCs, as gated on the contour plots (right) (n = 8).(F) Absolute numbers of CD11bhi Mo-Macs and KCs, as in E.(G) Histograms (top) and bar graphs (bottom) of MerTK, Clec4F, Tim4, Vsig4, FR-β, and CD163 expression in KCs, as in E.(H) Representative contour plots of CD206 and MHC-II expression in brain CD45+Lin−Ly6C−F4/80+CD64+ cells (left) and percentages of MG and BAM, as gated on the contour plots (right) (n = 6–8).(I) Absolute numbers of MG and BAM, as in H.(J) Histograms (top) and bar graphs (bottom) of P2RY12 and FCRLS expression in MG, as in H.(K) Representative contour plots of CD206 and MHC-II expression in BAM (left) and percentages of MHC-II− and MHC-II+ BAM, as gated on the contour plot (right) (n = 6–8).(L) Absolute numbers of MHC-II− and MHC-II+ BAM, as in K.(M) Representative contour plots of CD206 and CD11b expression in spleen CD45+Lin−Ly6C−F4/80+CD64+ cells (left) and percentages of RPM and CD11b+ mac, as gated on the contour plots (right) (n = 6–7).(N) Absolute numbers of RPM and CD11b+ macs, as in M.(O) Histograms (top) and bar graphs (bottom) of CD163 expression in RPM, as in M.Data are mean ± SEM and are pooled from two independent experiments. P values were calculated using a two-tailed Student’s t test. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. ns, not significant.See also Figures S4 and S5.