Immunohistochemical analysis of of paraffin-embedded human breast tissue using O8057-07. Tissue was fixed with formaldehyde at RT. Heat induced epitope retrieval was performed by EDTA buffer, pH 9. 0. Samples were incubated with O8075-07 (1:100) for 1 hour at RT. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Confocal Immunofluorescence analysis of in A375 cells using O8057-07 followed by Alexa Fuor 488-conjugated goat anti-rabbit IgG (green). DAPI was used to stain the cell nuclear (blue).

Overlay histogram showing Hela cells stained with O8057-07 (green line). The cells were fixed with 2% paraformaldehyde (10mins) and then permeabilized with 90% methanol for 10mins. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by O8057-07 (1:25) for 60mins at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 for 40mins at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10e6 cells) used under the same conditions. Acquisition of >10,000 events performed.