Western blot analysis of hypo- or hyper-acetylated recombinant p300 HAT domains, either wild-type or K1499R mutant, using C2098-10(upper). Also shown in the corresponding coomassie stained SDS-PAGE gel (lower). (Details are described in Thompson, P. A. et al. (2004) Nat. Struct. Mol. Biol. 11, 308-315.)