Western Blot analysis of extracts from HeLa cells transfected with 100nM Control siRNA (Unconjugated) (-) or c-Myc siRNA I (+) using 376932 (upper) or beta-Actin (lower). The c-Myc confirms silencing of c-Myc expression, while the beta-Actin is used as a loading control.

Western Blot analysis of extracts from HT-29 cells, untreated (-) or treated with JQ1 (1uM; 72 hr; +) using 346932 (upper) and beta-Actin (lower). As expected, the BET bromodomain inhibitor JQ1 inhibits c-Myc expression.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4x10e6 Daudi cells and 10ul of c-Myc, using Enzymatic Chromatin IP Kit (Magnetic Beads). DNA Libraries were prepared from 5ng enriched ChIP DNA using UltraTM II DNA Library Prep Kit, and sequenced on the Illumina NextSeq. The figure shows binding across chromosome 5 (upper), including NPM1 (lower), a known target gene of c-Myc (see additional figure containing ChIP-qPCR data).