Western blot analysis of HT-29 cells, untreated (-) or treated with combinations of the following treatments as indicated: Z-VAD (20uM, added 30 min prior to other compounds; +), human TNF-a (hTNF-a, 20ng/ml, 7 hr; +), SM-164 (100nM, 7 hr; +), and necrostatin-1 (Nec-1, 50uM, 7 hr; +), using Phospho-RIP (Ser166) Rabbit mAb (upper) or B-Actin Rabbit mAb (lower).