Flow cytometric analysis of HT-1080 cells using S1014-53A3 (blue) compared to a Rabbit mAb IgG XP Isotype Control (red).

Confocal immunofluorescent analysis of HT-1080 cells, serum-starved (left), treated with Human TGF-B1 (1ug/ml for 30 min, center) or treated with Human TGF-B1 and SB43152 (10ug/ml for 30 min, right), using S1014-53A3 (green). Actin filaments were labeled with DY-554 phalloidin (red) .

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4x10e6 HaCat cells treated with Human TGF-B3 (7ng/ml) for 1h and either 10ul of S1014-53A3 or 2ul of Normal Rabbit IgG using SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) The enriched DNA was quantified by real-time PCR using SimpleChIP Human CDKN1A Intron 1 Primers SimpleChIP Human ID1 Promoter Primers and SimpleChIP Human a Satellite Repeat Primers The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one .