Western blot analysis of extracts of various cell lines, using 211777 at 1:1000 dilution. HeLa cells were treated by Hydroxyurea (4mM) at 37°C for 20 hours or treated by Paclitaxel (100nM/ml) at 37°C for 20 hours. C6 cells were treated by Hydroxyurea (4mM) at 37°C for 20 hours or treated by Paclitaxel (100nM) at 37°C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 5s.