Formalin-fixed paraffin-embedded wilms tumor 1 tissue stained using Enzo’s WT1 Recombinant monoclonal antibody. IHC staining was performed on an automated Leica BOND III.

Anti-Utf1 Antibody (C-terminal) at 1:8000 dilution + mouse heart lysates Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution Predicted band size: 36kD Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Utf1 Antibody (C-terminal) at 1:2000 dilution + mouse liver lysates Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution Predicted band size: 36kD Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-Utf1 Antibody (C-terminal) at 1:1000 dilution + mouse testis lysates Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Predicted band size: 36kD Blocking/Dilution buffer: 5% NFDM/TBST.