Western blot analysis of extracts of A-431 cells, using Phospho-STAT5-Y694 pAb at 1:1000 dilution or STAT5B antibody .A-431 cells were treated by EGF(100ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit Exposure time: 60s.

Immunohistochemistry of paraffin-embedded rat heart using Phospho-STAT5-Y694 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded human placenta using Phospho-STAT5-Y694 antibody at dilution of 1:200 (40x lens).

Immunohistochemistry of paraffin-embedded mouse liver using Phospho-STAT5-Y694 antibody at dilution of 1:200 (40x lens).