Blot of bovine retinal extracts probed with 031425. The antibody stains a band corresponing to retinal rhodopsin at about 35kD. Bands about 70kD and 140kD are aggregated forms of rhodopsin. Note, due to the highly hydrophobic nature of rhodopsin, it important to avoid boiling samples containing this protein it in SDS-PAGE sample buffer, as this will result in even more extensive aggregation of the rhodopsin protein and appearance of more of this high molecular weight material.