Immunohistochemistry analysis of paraformaldehyde-fixed, paraffin-embedded human hepatic carcinoma using M3886-75A followed by peroxidase-conjugated secondary antibody and DAB staining. This data demonstrates the use of M386-75A for immunohistochemistry.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling MICA with M3886-75A (1;25) followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody (1;200) (green). Immunofluorescence image showing cytoplasm staining on Hela cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (1:100) (red).The nuclear counter stain is DAPI (blue).

Flow Cytometric analysis of SK-Br-3 cells using M3886-75A (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.