Human whole blood was fixed, lysed, and permeabilized as per the Flow Alternate Protocol and stained using NTAL/LAB (D7I2B) Rabbit mAb. Samples were co-stained with CD19-APC and CD3-PE to identify B and T cell populations, respectively. The forward/side scatter lymphocyte gate and CD19+ B cell population gate were combined and applied to a histogram depicting the mean fluorescence intensity of NTAL/LAB (blue) and a concentration-matched isotype control (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody.

Confocal immunofluorescent analysis of SR (positive; left) and Jurkat (negative; right) cells using NTAL/LAB (D7I2B) Rabbit mAb (green). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).