Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling STAT5A with STAT5A at 1/25 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (1583138) secondary antibody at 1/400 dilution (green). Confocal image showing nuclear staining on Hela cell line. Cytoplasmic actin is detected with Alexa Fluor 555 conjugated with Phalloidin (OB16636430) at 1/100 dilution (red).