Western blot analysis of human and mouse cell lines using 045085. Method: Total protein extracts (20ug) from various human (293-T, Jurkat, Raj, Ramos, BJAB, THP-1, U937, K562, Raw, HeLa) and mouse (EL-4, A20) cell lines were run on SDS-PAGE and NLRP1/NALP1 detected using 045085 at 1:500 dilution. Anti-rabbit IgG coupled horseradish peroxidase was used at 1:5000 dilution for ECL detection.