Western blot analysis of extracts from HeLa, C2C12, and C6 cells, untreated (-) or treated with chloroquine (50uM, overnight; +), using 351816 (upper) and b-Actin Rabbit mAb (lower).

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with chloroquine (50uM, 24 hr; right), using 351816 (green) and b-Actin Mouse mAb (red). Blue pseudocolor (fluorescent DNA dye).

Flow cytometric analysis of HeLa cells using 351816 (blue) compared to concentration-matched Rabbit mAb IgG Isotype Control (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) was used as a secondary antibody.