Western Blot analysis of 293T/17 cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with 41kD HA tagged vector using 036504.

Western Blot analysis of lysate from 12 tag recombinant protein cell line, using 036504 (1:1000). An IgG H&L (HRP) goat anti-rabbit (1:5000) was used as the secondary antibody. Lysate at 35ug.

ChIP was performed with 35S:HATCL1 plants using anti-HA antibodies. Rabbit preimmune serum was used as a mock control. Primer sets specific for the first intron or the 3_x0004_-UTR region of GL1 were used in PCR reactions. ACTIN2 provided a control(Provided by Shucai Wang, Su-Hwan Kwak:Development 134, 3873-3882 (2007))

GTPase activity of dynamin-2 is required for endocytosis of cell-surface tTG. MRC-5 fibroblasts were transiently transfected with either wild-type (wt) or a GTPase-deficient dynamin-2 mutant (K44A) with a hemagglutinin (HA) tag.(Provided by Evgeny A. Zemskov, Irina Mikhailenko:Journal of Cell Science 120, 3188-3199 (2007))