Western blot analysis using C8453-07 shows detection of Cyclin B1 present in asynchronous HeLa cell lysates. ~50ug of lysate was loaded on to a 7% SDS-PAGE gel for separation. After transfer to nitrocellulose, the blot was incubated with a 1:500 dilution of C8453-07 for 1hr at RT. Detection occurred using I1904-39.

C8453-07 was diluted 1:500 to detect Cyclin B1 in human brain cerebellum tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of C8453-07 as the precipitated red signal, with a hematoxylin purple nuclear counter stain.