Western Blot analysis in U-87 MG whole cell lysate (20ug) using 037534 (1:2000). Secondary IgG, (H+L) (HRP) goat anti-rabbit at 1:10,000. Predicted band size: 27kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Western Blot analysis in WiDr cell line lysates (35ug/lane) using 037534. This demonstrates that 037534 detected the KLF9 protein (arrow).

Fluorescent confocal image of U251 cell stained with 037534. U251 cells were fixed with 4% PFA (20mins), permeabilized with Triton X-100 (0.1%, 10mins), then incubated with 037534 (1:25, 1hr at 37ºC). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50mins at 37ºC). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1hr at 37ºC). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). KLF9 immunoreactivity is localized to vesicles and Nucleus significantly.