Anti-Phospho-KDR/FLK1-Y996 Pab is used in Western blot to detect Phospho-KDR/FLK1-Y996 in HMVEC cell line lysate. Endothelial cells were stimulated with 50ug/ml VEGF for 5min; 20ug lysate from HMVEC was loaded onto an 8% gel; for Western blot, membranes were incubated O/N with Phospho-KDR/FLK1-Y996 Antibody (AP3107a)diluted to 1:500 in 1% Milk/TBST. Data and Protocol kindly provided by Dr. Weis from Cheresh Lab, UCSD.