Western blot analysis of human tau (2N4R) using various antisera. Lane 1: MW markers; Lanes 2 and 3: Tau1 (control); Lanes 2, 4 and 6: unphosphorylated tau; lanes 3, 5 and 7: p56lck phosphorylated tau; ~400ng tau per lane. Lanes 4 and 5: antiphosphotyrosine (4G10) (1:1000); Lanes 6 and 7: antiserum TA 3119 (1:3000). Enhanced chemiluminescence (1 min exposure).

Anti-RB(S612) Antibody at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 106kD Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-RB(S612) Antibody at 1:2000 dilution + Jurkat whole cell lysate Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution. Predicted band size: 106kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of RB on paraffin-embedded Human retina tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9.0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.