Western blot analysis of hnRNP A2B1 using hnRNP A2B1. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: human SW620 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human 22RV1 whole cell lysates, Lane 7: human A431 whole cell lysates, Lane 8: human A375 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with hnRNP A2B1 at 0.5ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for hnRNP A2B1 at approximately 37kD. The expected band size for hnRNP A2B1 is at 37kD.