Western Blot analysis in HT-29 whole cell lysate (20ug/lane) using 037592 (1:2000). Secondary: IgG (HRP) Goat Anti-Rabbit (1:10,000). Predicted band size: 22kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Western Blot analysis using 037592 (1:500). Lane 1: Hela whole cell lysate Lane 2: 293T/17 whole cell lysate Lane 3: C2C12 whole cell lysate Lane 4: C6 whole cell lysate Lane 5: HT-29 whole cell lysate Lane 6: PC-12 whole cell lysate Lysates/proteins: 20ug/lane. Secondary: IgG (HRP) Goat Anti-Rabbit, (1:10,000). Predicted band size: 22kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Western Blot analysis using 037592 (1:2000) Lane 1: 293T/17 whole cell lysate Lane 2: C2C12 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: HT-29 whole cell lysate Lane 5: K562 whole cell lysate Lane 6: PC-12 whole cell lysate Lane 7: Ramos whole cell lysate. Lysates/proteins: 20ug/lane. Secondary: IgG (HRP) Goat Anti-Rabbit 1:10,000. Predicted band size: 22kD. Blocking/Dilution buffer: 5% NFDM/TBST.
Confocal immunofluorescent analysis of WiDr cells using 037592 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Flow Cytometric analysis of Hela cells using 037592 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Supplier Page from United States Biological for Rabbit Anti-KRAS, CT Antibody