Lane labelled CBB shows Coomassie Brilliant Blue stained crude extract of rat spinal cord, with the prominent major neurofilament subunit indicated; In rodents, NF-H runs at 200kD, NF-M at 145kD and NF-L at 68kD. The Rab lane shows a blot probed with rabbit anti NF-H antibody 031398. A prominent band at an apparent SDS-PAGE molecular weight of 200kD corresponds to phosphorylated form of NF-H. The lane marked Rab shows a similar blot probed with our rabbit antibody to NF-H, 031399.