Western Blot analysis using 043900 (1:2000) Lane 1: K562 whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: HT-1080 whole cell lysate. Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit (1:10,000) Predicted band size: 49kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of formalin fixed paraffin embedded human kidney tissue using 04390 followed by Peroxidase-conjugated antibody and DAB staining.

Confocal immunofluorescent analysis with MCF-7 cell using 043900 and Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nucleus (blue).

Flow Cytometry analysis of MCF-7 cells using 043900 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.