Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody 168475 from United States Biological

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Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

Western Blot analysis of RFP recombinant protein using 168475 1:2500 for 1 h at RT followed by washes and reaction with IRDye™800 conjugated Goat-a-Rabbit IgG [H&L] . Lane 1 shows no reaction against a GFP recombinant protein present in 10ug of HeLa cell extract. Lane 2 shows a single band detected in 10ug of a HeLa lysate containing RFP recombinant protein as a 27kD band.
Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

Western Blot analysis using 168475 Pre-Absorbed (1:1000) overnight at 4°C. Lane 1: RFP Lane 2: Human IgG Lane 3: Goat IgG Lane 4: Mouse IgG Load: 50ng per lane. Secondary antibody: Peroxidase conjugated rabbit secondary antibody for 30 min at RT. Block: Blocking Buffer for 30 min at RT. Predicted/Observed size: 27kD, 30kD
Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

Immunofluorescence Microscopy analysis of mouse lung tissue (10X) using 168475 (1:50) for 1h at RT. Fixation: 4% PFA. Antigen retrieval: Heat. Secondary antibody: Fluorescein rabbit secondary antibody for 1 hr at RT. Staining: SPC+ cells are RFP positive in red.
Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

Immunofluorescence Microscopy analysis of mouse E14.5 embryo heart tissue (10X) using 168475 (1:50) for 1h at RT. Fixation: 4% PFA. Antigen retrieval: Heat. Secondary antibody: Fluorescein rabbit secondary antibody for 1 hr at RT. Staining: cardiac cells are RFP positive in red in tomato transgenic mice.
Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

Immunofluorescence Microscopy analysis of HopERCre/+; R26Tom/+ mice using 168475 (10ug/ml) for 1h at RT. Fixation: 0.5% PFA. Antigen retrieval: Tamoxifen. Secondary antibody: Fluorescein rabbit secondary antibody for 45 min at RT. Localization: RFP is nuclear and occasionally cytoplasmic. Staining: Hop-derived cells in the hair follicle, labeled in red.

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United States Biological for
Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

The Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody from United States Biological is a Rabbit Polyclonal antibody. The Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody has been shown to work in the following applications: EIA, Immunoassay, ELISA, Flow Cytometry, Immunohistochemistry, and Western Blot.

Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody

Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody from United States Biological

United States Biological

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Pab Rb x Red Fluorescent Protein, Pre-adsorbed (RFP) antibody from United States Biological

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