Western Blotting Analysis: Representative lot data. HeLa cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-14R1 (1:4000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates dimethyl Histone H3 (~17kD)