Proximity Ligation Analysis (PLA) of protein-protein interactions between VASP and ACTG1. HeLa cells were stained with VASP rabbit purified polyclonal 1:1200 and ACTG1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.