Immunocytochemistry Analysis: Representative lot data. Confocal fluorescent analysis of non-transfected CHEM1 cells, CHEM1 cells transfected with LPA receptor 5, and TF1 cells using Anti-LPA receptor 5 (GPR92) and a Donkey Anti-Rabbit secondary antibody conjugated to Cy3 (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye - Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the cytoplasm. NOTE: The protein used to make the transfected CHEM1 cell line is full length LPAR5.
Western Blotting Analysis: Representative lot data. K562 cell lysate was probed withnti-LPA receptor 5 (GPR92) (0.2ug/ml). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates LPA Receptor 5 ( ~37kD).
Supplier Page from United States Biological for Rabbit Anti-LPAR5 Antibody (HRP)