Immunohistochemistry analysis of paraffin-embedded human lung section using 041642 (1:200). An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized A549 cells labeling SFTPC using 041642 (1:25) followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody (1:200) (green). Immunofluorescence image showing cytoplasm staining on A549 cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (1:500) (red). The nuclear counter stain is DAPI (blue).

Overlay histogram showing A549 cells stained using 041642 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by 041642 (1:25) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed (1:200) for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.