Western Blot analysis using 043892 (1:500). Lane 1: Hela whole cell lysate Lane 2: Mouse brain lysate Lane 3: Mouse heart lysate Lane 4: PANC-1 whole cell lysate Lysates/proteins at 20ug/lane. Secondary: IgG (HRP) goat Anti-rabbit (1:10,000). Predicted band size: 42kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of formalin fixed paraffin embedded human lung carcinoma using 043892 followed by peroxidase conjugated secondary antibody and DAB staining. This data demonstrates the use of 043892 for immunohistochemistry.

Immunohistochemistry analysis of formalin fixed paraffin embedded human thyroid carcinoma tissue performed on the Leica® BOND RXm. Antigen retrieval was by heat mediation with a EDTA buffer (pH 9. 0) followed using 043892 (1:100). An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Flow Cytometric analysis of Hela cells using 043892 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.