Western blot analysis of extracts from L-540 cells, untreated or pre-incubated with either a site-specific phosphorylated peptide to block the signal or a site-specific non-phosphorylated peptide, using Phospho-TNK1 (Tyr277) Rabbit mAb. L-540 cells express a 58kD TNK1-C17orf61 fusion protein containing 466 amino acids from the amino terminus of TNK1 [Gu, T.L. et al. (2010) Leukemia].