Anti-atg5 Antibody (Center) at 1:4000 dilution + NIH/3T3 whole cell lysates Lysates/proteins at 20ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10,000 dilution Predicted band size: 32kD Blocking/Dilution buffer: 5% NFDM/TBST.
Supplier Page from United States Biological for Pab Rb x human Atg5, ID antibody