Pab Rb x human SMARCA4 (Transcription Activator BRG1, TP-Dependent Helicase, BRG1-Associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-Beta, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin Subfamily A Member 4, BAF190A, BRG1, SNF2B, SNF2L4) antibody 397555 from United States Biological

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Pab Rb x human SMARCA4 (Transcription Activator BRG1, TP-Dependent Helicase, BRG1-Associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-Beta, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin Subfamily A Member 4, BAF190A, BRG1, SNF2B, SNF2L4) antibody

Figure 1. Western blot analysis of BRG1 using BRG1. Electrophoresis was performed on a 5-20% SDS- PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: human A431 whole cell lysates, Lane 6: human SGC-7901 whole cell lysates, Lane 7: human 22RV1 whole cell lysates, Lane 8: rat small intestine tissue lysates, Lane 9: mouse small intestine tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with BRG1 at 0.5ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BRG1 at approximately 220kD. The expected band size for BRG1 is at 185kD.
Pab Rb x human SMARCA4 (Transcription Activator BRG1, TP-Dependent Helicase, BRG1-Associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-Beta, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin Subfamily A Member 4, BAF190A, BRG1, SNF2B, SNF2L4) antibody

Figure 2. Immunohistochemistry analysis of BRG1 using BRG1. BRG1 was detected in paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit BRG1 Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin- Complex (SABC) with DAB as the chromogen.
Pab Rb x human SMARCA4 (Transcription Activator BRG1, TP-Dependent Helicase, BRG1-Associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-Beta, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin Subfamily A Member 4, BAF190A, BRG1, SNF2B, SNF2L4) antibody

Figure 3. Immunohistochemistry analysis of BRG1 using BRG1. BRG1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml BRG1 Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin- Complex (SABC) with DAB as the chromogen.
Pab Rb x human SMARCA4 (Transcription Activator BRG1, TP-Dependent Helicase, BRG1-Associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-Beta, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin Subfamily A Member 4, BAF190A, BRG1, SNF2B, SNF2L4) antibody

Figure 4. Immunohistochemistry analysis of BRG1 using anti-BRG1. BRG1 was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml BRG1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin- Complex (SABC) with DAB as the chromogen.

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Pab Rb x human SMARCA4 (Transcription Activator BRG1, TP-Dependent Helicase, BRG1-Associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-Beta, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin Subfamily A Member 4, BAF190A, BRG1, SNF2B, SNF2L4) antibody

The Pab Rb x human SMARCA4 (Transcription Activator BRG1, TP-Dependent Helicase, BRG1-Associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-Beta, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin Subfamily A Member 4, BAF190A, BRG1, SNF2B, SNF2L4) antibody from United States Biological is a Rabbit Polyclonal antibody. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Pab Rb x human SMARCA4 (Transcription Activator BRG1, TP-Dependent Helicase, BRG1-Associated Factor 190A, BAF190A, Mitotic Growth and Transcription Activator, Protein BRG-1, Protein Brahma Homolog 1, SNF2-Beta, SWI/SNF-Related Matrix-Associated Actin-Dependent Regulator of Chromatin Subfamily A Member 4, BAF190A, BRG1, SNF2B, SNF2L4) antibody has been shown to work in the following applications: EIA, Immunoassay, ELISA, Immunohistochemistry, and Western Blot.

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