Western Blot analysis of MOLT-4 whole cell lysate (20ug/lane) using U4100-55B (1:1000). Secondary: IgG (HRP) Goat Anti-Rabbit (1:10,000). Predicted band size: 112kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of paraffin-embedded human epityphlon tissue using U4100-55B (1:2500) performed on the Leica® BOND RXm. Samples were incubated with U4100-55B (1:2500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemistry analysis of paraffin-embedded human liver tissue using U4100-55B (1:500) performed on the Leica® BOND RXm. Samples were incubated with U4100-55B (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunofluorescent analysis of USP15 of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized Hela cells using using U4100-55B (1:25) followed by Dylight® 488-conjugated goat anti-Rabbit IgG secondary antibody at (1:200) (green). Immunofluorescence image showing Cytoplasm and Weak Nucleus staining on Hela cell line. The nuclear counter stain is DAPI (blue).