Mouse neutrophils are depleted in vivo by anti-Neutrophils (mouse), mAb (blocking) Method: Mice were injected) i.p. with 250µg of anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (B) or with Control mAb (A) in BALB/c mouse 6 h prior to Leishmania major infection (3x106 parasites injected in the hind footpad). 3 days later, blood (100µl) was subjected to flow cytometric analysis after staining with APC/CY7-labeled anti-Ly6G antibody (clone 1A8).