Western Blot of 168289. Lane A: Human brain tissue lysate in the absence of blocking peptide. Lane B: Human brain tissue lysate in the presence of blocking peptide. Primary antibody: 168289 (0.25ug/ml). Secondary antibody: Peroxidase rabbit secondary antibody (1:10,000) for 45 min at RT. Block: 5% BLOTTO overnight at 4°C.