Western blot analysis of extracts from normal (control) and TFAP4 knockout (KO) HeLa cells, using TFAP4 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit Exposure time: 60S.

Immunofluorescence analysis of C6 cells using TFAP4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U-2 OS cells using TFAP4 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.