Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody 397493 from United States Biological

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Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody

Figure 1. Western blot analysis of NFIB/NF1B2 using NFIB/NF1B2. Electrophoresis was performed on a 5-20% SDS- PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: rat PC-12 whole cell lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse ovary tissue lysates, Lane 5: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with NFIB/NF1B2 at 0.5ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NFIB/NF1B2 at approximately 50kD. The expected band size for NFIB/NF1B2 is at 47kD.
Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody

Figure 2. Immunohistochemistry analysis of NFIB/NF1B2 using NFIB/NF1B2. NFIB/NF1B2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit NFIB/NF1B2 Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody

Figure 3. Immunohistochemistry analysis of NFIB/NF1B2 using NFIB/NF1B2. NFIB/NF1B2 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml NFIB/NF1B2 Antibody overnight at 4°C. Biotinylated goat rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin- Complex (SABC) with DAB as the chromogen.
Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody

Figure 4. Immunohistochemistry analysis of NFIB/NF1B2 using anti- NFIB/NF1B2. NFIB/NF1B2 was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml NFIB/NF1B2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

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Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody

The Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody from United States Biological is a Rabbit Polyclonal antibody to NFIB, and nuclear factor I B. This antibody recognizes Homo Sapiens, Human, Mouse, and Rat antigen. The Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody has been shown to work in the following applications: Immunohistochemistry, and Western Blot.

Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody

Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody

Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody

Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody

Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody from United States Biological

United States Biological

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Pab Rb x human NFIB (Nuclear Factor 1 B-Type, NF1-B, Nuclear Factor 1/B, CCAAT-Box-Binding Transcription Factor, CTF, Nuclear Factor I/B, NF-I/B, NFI-B, TGGCA-Binding Protein) antibody from United States Biological

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