Western blot analysis of CDCA7L/RAM2 in HCT-116 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using J5550-50A at 4ug/ml and 2ug/ml, respectively. Goat anti-rabbit Ig HRP secondary antibody and ECL substrate solution were used for this test.