Western Blot analysis of extracts from THP-1, RL7, and A20 cell lines using I7662-93G.

Western Blot analysis of extracts from 293T cells, mock transfected or transfected with human and mouse IRF-8 constructs, using I7662-93G.

Chromatin immunoprecipitations were performed with cross-linked chromatin from 4x10e6 THP-1 and either 20ul of I7662-93G or 2ul of Normal Rabbit IgG using SimpleChIP Enzymatic Chromatin IP Kit (Magnetic Beads) The enriched DNA was quantified by real-time PCR using human GPR18 promoter primers, human MS4A7 promoter primers, and SimpleChIP Human a Satellite Repeat Primers The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.