Western Blot analysis of RNF14 expression in transfected 293T cell line by RNF14 monoclonal antibody . Lane 1: RNF14 transfected lysate (39.6kD). Lane 2: Non-transfected lysate.

Immunofluorescence of monoclonal antibody to RNF14 on HeLa cell . [antibody concentration 10ug/ml

Detection limit for recombinant GST tagged RNF14 is ~0.1ng/ml as a capture antibody.

Western blot analysis of RNF14 over-expressed 293 cell line, cotransfected with RNF14 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RNF14 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.