Western Blot analysis using 042054 (1:4000) Lane 1: K562 whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: HepG2 whole cell lysate Lane 4: A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary: IgG (HRP) Goat Anti-Rabbit (1:10000) Predicted band size : 70kD Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of formalin fixed paraffin embedded human testis tissue using 042054 (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica® BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human breast tissue using 042054 (1:500) after HIER using EDTA buffer pH9.0 prior to staining performed on the Leica® BOND RXm. An undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.