Mab Rb x human Lymphotoxin alpha antibody 516745 from United States Biological

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Mab Rb x human Lymphotoxin alpha antibody

Flow cytometry using the anti-Lymphotoxin alpha antibody 3F12.2D3 (Ab00788) U937 cells were fixed using 2% PFA, permeabilised using 0.5% Triton and stained with unimmunized rabbit IgG antibody (MOPC-21; isotype control, black line) or the rabbit IgG-chimeric version of 3F12.2D3 (blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow cytometry.
Mab Rb x human Lymphotoxin alpha antibody

Western Blot using anti-Lymphotoxin alpha antibody 3F12.2D3 (Ab00788) Lipopolysaccharide(LPS)-treated human peripheral blood monocyte (PBM) lysate (35ug protein in RIPA buffer) was resolved on a 15% SDS PAGE gel and blots probed with the rabbit IgG-chimeric version of 3F12.2D3 at 2ug/ml before detection using an anti-rabbit secondary antibody. Protein was detected by chemiluminescence. The expected unmodified running size for Lymphotoxin alpha is 22.3kD, though the form shown here is likely glycosylated. Successfully detected Lymphotoxin alpha in LPS-treated human PBM samples.

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Mab Rb x human Lymphotoxin alpha antibody

The Mab Rb x human Lymphotoxin alpha antibody from United States Biological is a Rabbit Monoclonal antibody to LTA, and Lymphotoxin alpha. This antibody recognizes Homo Sapiens, and Human antigen. The Mab Rb x human Lymphotoxin alpha antibody has been shown to work in the following applications: EIA, Immunoassay, ELISA, and Flow Cytometry.

Mab Rb x human Lymphotoxin alpha antibody

Mab Rb x human Lymphotoxin alpha antibody

Mab Rb x human Lymphotoxin alpha antibody from United States Biological

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Mab Rb x human Lymphotoxin alpha antibody from United States Biological

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