Western Blot using anti-CD74 antibody In-1 (Ab00876) Mouse spleen (A) and mouse lymph node (B) lysate samples (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit version of In-1 at 0.0001ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for unmodified CD74 is 31.6kD, and this protein is glycosylated at several residues. Successfully detected CD74 in mouse spleen and mouse lymph node lysates.