Immunohistochemistry of paraffin-paraformaldehyde-fixed, paraffin-embedded sections of human liver tissue sectionsusig 501860. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at RT; antigen retrieval was by heat mediation with a citrate buffer, pH 6. Samples were incubated with 501860 for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.