Immunohistochemical analysis of paraffin-embedded H.kidney section using DFFB Antibody (Center). DFFB was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Flow cytometric analysis of Hela cells using DFFB Antibody (Center)(green) compared to an isotype control of rabbit IgG(blue). DFFB was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.

Western blot analysis of lysate from A431 cell line, using DFFB Antibody (Center). DFFB was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.