Western Blot analysis using T1041-04G (1:2000): Lane 1: rat brain lysate (20ug) Lane 2: mouse brain lysate (20ug) Secondary antibody: IgG (H&L) (HRP) goat anti-rabbit Blocking/dilution buffer: 5% NFDM/TBST.

Immunohistochemistry analysis of Cynomolgus monkey brain tissue using T1041-04G. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5hr at RT. HIER using citrate buffer (pH6) was used. Samples were incubated with T1041-04G (1:25) for 1hr at 37°C. Biotinylated goat anti-rabbit polyclonal IgG was used as secondary antibody.

Confocal immunofluorescent analysis of MCF-7 cells using T1041-04G. Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) was used to visualize the antigen. DAPI was used to stain the cell nuclear (blue).