Immunohistochemistry analysis of paraffin embedded human hepatocarcinoma using 036741. Tissue was fixed with formaldehyde at RT. Aantigen retrieval was by heat mediation with a EDTA buffer pH 9.0. Samples were incubated with 036741 (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemistry analysis of paraffin embedded human colon tissue using 036741. Tissue was fixed with formaldehyde at RT. Aantigen retrieval was by heat mediation with a EDTA buffer pH 9.0. Samples were incubated with 036741 (1:500) for 1 hours at RT. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Confocal immunofluorescent analysis of MDA-MB435 cells using 036741 followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Indirect Flow Cytometric analysis of MDA-MB435 cells using 036741 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.