Confocal fluorescent analysis of A431 and HeLa cells using H5110-14J (Red). Actin filaments have been labeled with Alexa Fluor® 488 dye-Phalloidin (Green). Nucleus is stained with DAPI (Blue). This antibody positively stains the nucleus.

Western Blot Analysis: HeLa acid extract (lane 1) and recombinant Histone H3 (lane 2) were probed with a 1:500 dilution of H5110-14J. Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP a chemiluminescence detection system. Arrow indicates dimethyl-Histone H3 (Lys4) (~17kD). An uncharacterized band at ~40kD may be observed in some cell lysates.