All lanes: 035249 (1:1000) Lane 1: Jurkat whole cell lysates Lane 2: 293T whole cell lysates. Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), peroxidase conjugated at 1:10,000. Predicted band size: 50kD. Blocking/Dilution buffer: 5% NFDM/TBST.

Flow Cytometric analysis of Jurkat cells using 035249 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Confocal immunofluorescent analysis with MCF-7 cells using 035249 followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).